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Accueil > Actualités

Salle de réunion bat. LWOFF

Lundi 12/01/15 - 11h00

David Bickard (Inst. Pasteur)

Titre : Studying and fighting bacteria with the help of CRISPR
CRISPR loci and the associated Cas genes are the adaptive immune system of archeae and bacteria. The Cas9 protein, a RNA-guided nuclease from the S. pyogenes CRISPR system, can be used as a tool for genome engineering. The approach relies on the ability to re-program CRISPR specificity through a small guide RNA, which directs Cas9 to cleave a target genomic locus. This selects for the recombination of a homologous template containing a desired mutation. This method allows the easy engineering of any genomic loci in a diversity of bacteria. The catalytic site of Cas9 can also be mutated, giving dCas9 (dead Cas9), and repurposing it as a RNA-guided DNA binding protein. This binding is strong enough to block transcription and can thus be used to easily knock-down any gene in the cell. Another area of focus is the development of sequence-specific antimicrobials using CRISPR. When directed to cleave the host cell genome, the Cas9 nuclease leads to cell death. Phagemids can be used as vectors to deliver self-targeting CRISPR systems to bacterial populations. The CRISPR can be programmed to kill bacteria carrying antibiotic resistance or virulence genes, leaving the rest of the microbiota intact.