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Home > Research teams > Chromatin and Regulation of bacterial Pathogenesis > Non-coding RNA

Non-coding RNA

Bacterial noncoding RNA (ncRNA) are small molecules with a regulatory rôle in the cell. They act in particular at the post-transcriptional level, and are able to activate or repress target messenger RNAs by using the complementarity of their respective sequence. Modeling suggests that this regulation is favorable when the bacterium must quickly regulate these genes’ expression in response to environmental variations. We postulate that these ncRNAs are involved in the fast adaptation of phytobacteria. We undertook the identification of the ncRNA in Dickeya dadantii and Agrobacterium tumefaciens, and we study the cellular functions of the candidates. To this aim, we use an interdisciplinary approach combining bioinformatics and experimental analyses.

Non-coding RNAs interact with mRNAs though base-pairing
Non-coding RNAs interact with mRNAs though base-pairing
A- Antisense RNAs are transcribed in the opposite strand of their target, showing a complete complementary of sequence. B- Other non-coding RNA genes are present in intergenic regions, showing limited complemetarity with their multiple target mRNAs transcribed elsewhere in the genome. They are called trans-acting RNAs.
RNA-RNA interactions modulated mRNA stability and/or mRNA translation
RNA-RNA interactions modulated mRNA stability and/or mRNA translation
A- Impact on mRNA stability: RNA-RNA interactions could mask or release specific site of RNA cleavages leading to an activator or a repressor effect, respectively. B- Impact on translation: RNA-RNA interaction could release or mask the Ribosome Binding Site (RBS) leading to an activator or a repressor effect, respectively. The protein Hfq is usually involved to facilitate RNA-RNA interaction.
Tumors are symptoms caused by Agrobacterium tumefaciens
Tumors are symptoms caused by Agrobacterium tumefaciens
A. tumefaciens infection on Kalanchoé (top on left) or on tomato (top on right) cause an anarchical proliferation of vegetal cells (or tumors). Pictures on the bottom illustrate results obtain with an A. tumefaciens mutant strain defected for the non-coding RNA RNA1111. As illustrated a decrease of the symptom is observable on Kalanchoé and on tomato.