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Home > Research teams > Chromatin and Regulation of bacterial Pathogenesis > Transcriptional regulation networks and assembly of nucleoprotein complexes

Transcriptional regulation networks and assembly of nucleoprotein complexes

Regulation of bacterial gene expression by Transcription Factors is relatively well understood. On the other hand, how the different factors use the specific structural organisation of particular gene promoters to integrate their actions and adjust the transcription level remains an open question. Our objective is to provide original, detailed and exhaustive information on how changes in environmental conditions impact the bacterial virulence gene expression through the formation of higher-order nucleoprotein complexes. For this purpose, we use an integrative approach to genetic regulation, unifying several levels of organisational complexity by combining the classical methods of microbiology and biochemistry with computational modelling and microscopy. Thus, an important innovative aspect of our project lies in its transdisciplinary methodology. At a larger scale, we also develop a computational analysis of gene expression data, in order to infer gene regulatory networks. Quantitative models of the most relevant networks are set up by integrating both genetic and metabolic information. The combination of this approach with detailed molecular investigation of individual promoters constitutes an original multi-scale description of bacterial gene regulation.

Assembly of nucleoprotein complexes at gene promoters and their impact on gene expression
Nucleoprotein complex assemblies are studied using a combination of biochemical and biophysical methods, and molecular genetics. Computational methods are developed to infer regulation networks.