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ANR RegulPath, BQR INSA 2012

Global virulence response of Dickeya dadantii

 Global virulence response of Dickeya dadantii

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Jiang et al. 2016. Environ Microbiol.

Jiang et al. 2016. Global transcriptional response of Dickeya dadantii to environmental stimuli relevant to the plant infection." Environ Microbiol. 2016 Mar 3. doi : 10.1111/1462-2920.13267.

The successive stages of bacterial infection represent an array of distinct environmental conditions to which bacteria need to adapt. To understand the cellular response to environmental perturbations, it is important to determine the behaviour of cells on a systemic scale. This study focuses on gene expression profiling of the plant pathogen Dickeya dadantii response to various environmental stimuli encountered during infection. Dickeya species are soft rot disease-causing bacterial plant pathogens. An efficient colonization of the plant requires intense multiplication of bacteria. However, during infection, D. dadantii is confronted with various hostile environments in the intercellular apoplastic fluid : acidity, alkalinity, influx of Ca and exflux of K, reactive oxygen species, or ospmotic stress. For the firs time, we explore the specific response of D. dadantii to oxidative, acidic and osmotic stress, growth phase, pectin catabolism and the presence of plant extracts to build a comprehensive picture of the bacterial adaptative behaviour. We propose a temporal map of the bacterial response to various stress conditions and show that D. dadantii elicits complex genetic behaviour combining common stress-response genes with distinct sets of genes specifically induced under each particular stress. Comparison of our dataset with an in planta expression profile reveals the combined impact of stress factors and enables us to predict the major stress confronting D. dadantii at a particular stage of infection.

Voir en ligne : Jiang et al. @ Wiley