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FRM SPF20130526652, CNRS–INSERM ATIP-Avenir, Sanofi, Labex ECOFECT, ANR-11- IDEX-0007, ANR-10-BINF-01-01, NSERC, CIHR114975

Silencing of natural transformation in Legionella

Silencing of natural transformation in Legionella

 Silencing of natural transformation in Legionella

Competence group

Attaeich et al., 2016 PNAS

Attaiech, L., A. Boughammoura, C. Brochier-Armanet, O. Allatif, F. Peillard-Fiorente, R. A. Edwards, A. R. Omar, A. M. MacMillan, M. Glover, and X. Charpentier. 2016. Silencing of natural transformation by an RNA chaperone and a multitarget small RNA. Proceedings of the National Academy of Sciences : 201601626.
A highly conserved DNA uptake system allows many bacteria to actively import and integrate exogenous DNA. This process, called natural transformation, represents a major mechanism of horizontal gene transfer (HGT) involved in the acquisition of virulence and antibiotic resistance determinants. Despite evidence of HGT and the high level of conservation of the genes coding the DNA uptake system, most bacterial species appear non-transformable under laboratory conditions. In naturally transformable species, the DNA uptake system is only expressed when bacteria enter a physiological state called competence, which develops under specific conditions. Here, we investigated the mechanism that controls expression of the DNA uptake system in the human pathogen Legionella pneumophila We found that a repressor of this system displays a conserved ProQ/FinO domain and interacts with a newly characterized trans-acting sRNA, RocR. Together, they target mRNAs of the genes coding the DNA uptake system to control natural transformation. This RNA-based silencing represents a previously unknown regulatory means to control this major mechanism of HGT. Importantly, these findings also show that chromosome-encoded ProQ/FinO domain-containing proteins can assist trans-acting sRNAs and that this class of RNA chaperones could play key roles in post-transcriptional gene regulation throughout bacterial species.

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