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Hot off the press [Brève INSB]

Shih. et al. Nature Comm. 2016

When Bio-bricks go green (Plant Synthetic Biology)

Shih, P. M., K. Vuu, N. Mansoori, L. Ayad, K. B. Louie, B. P. Bowen, T. R. Northen, and D. Loque. 2016. A robust gene-stacking method utilizing yeast assembly for plant synthetic biology. Nat Commun 7 : 13215.

Lien vers brève INSB

The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs. However, plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. Here, we describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. Our approach provides a powerful alternative to conventional strategies for stacking genes and traits to address many impending environmental and agricultural challenges.

Keywords : Synthetic biology, Yeast recombination

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