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Accueil > Séminaires

Vendredi 9 février - 11h00

Séminaire de Martial MARBOUTY, Institut Pasteur, Paris.
"Characterization of (Meta)genomes structures and dynamics using high-throughput chromosome conformation capture data"

Salle de réunion bat. Lwoff, sous-sol.

Metagenome sequence analysis relies principally on compositional approaches, which hypothesise that sequences sharing similar characteristics (GC%, codon bias, coverage-covariation... etc.) Although these approaches have generated important results, they remain somehow limited and do not allow the full characterization and understanding of the genetic composition of a complex microbial population. Contact genomics, which aimed at exploiting the 3D physical signature of genomes to solve their sequence, has the potential to alleviate or improve some of these caveats. To explore the genomic content of bacterial population at a new level of resolution we have recently developed meta3C (Marbouty et al. 2014), a derivative protocol of the chromosome conformation capture (3C ; Dekker et al. 2002) assay that aims at deciphering the average 3D organization of a genome. Using controlled mixes of bacterial or yeast species, we showed that the frequent collisions experienced by DNA molecules sharing similar cellular compartment can be measured through meta3C and conveniently used to assemble larger scaffolds of the genomes present in a metapopulation.
Here i will present data obtained from different complex mixes of species of the gut microbiota of mouse (Marbouty et al. 2017). Meta3C allow unveiling hundreds of genomic compartments, hence species. Moreover, meta3C allow to link phage sequences to their bacterial host and provide a convenient way to study interactions between genomic entities in a complex population. I will discuss the different way to explore this network and the results in light of the promising potential of the approach for future applications.​​​

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